Dracula Path of the Dragon - Filament test
#614770
04/16/10 07:40 AM
04/16/10 07:40 AM
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Joined: Jun 2006
Posts: 515 London, UK
Merlin
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Posts: 515
London, UK
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Hello everyone  I've worked out the first parameter no problem for the 2 percentage numbers for simple and polynuclear granulocytes. But I'm stuck on working out how to do the second parameter of the filament blood test, getting the number of granulocytes per ml. I've read kruger's example about counting animals and marking them, but I can't see how I apply that. I tried emptying the filament and refilling it with the same sample, and then under the microscope it brings up some different numbers each time, am i meant to do that?
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Re: Dracula Path of the Dragon - Filament test
[Re: Merlin]
#614807
04/16/10 10:00 AM
04/16/10 10:00 AM
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Joined: Jul 2005
Posts: 23,997 UK
Rushes
True Blue Boomer
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True Blue Boomer
Joined: Jul 2005
Posts: 23,997
UK
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Hi Merlin, My memory of this puzzle is a little hazy. This is from an older post by MaG, I hope it helps you: Procedure for second parameter: This is explained by the last page of Kruger's instructions. Take the filament from the microscope with the marked cells and attach it back to top of the sampler. Lower the sampler using the lever at left. Press the - button to eject the marked cells back in the test tube. This mixes the marked cells with the unmarked cells. Lower the sampler using the lever at left. Press the + button to take a sample of the mixed marked and unmarked sample. Take the filament and place it on the microscope. -------Note the % marked granulocytes in the mixed sample. The icons should NOT be lit up. The readout is above the icons. Place the filament back on top of the sampler. Lower the sampler using the lever at left. Press the - button to eject the marked cells back in the test tube. It is now ready for the next sample. Once you get the number similar to the chart in the walkthrough. Then enter the Total # of granulocytes/ml on the second parameter/ If you still need help, let us know. 
"Bleat, Watson -- unmitigated bleat!" ~ Sherlock Holmes
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Re: Dracula Path of the Dragon - Filament test
[Re: Rushes]
#614825
04/16/10 11:01 AM
04/16/10 11:01 AM
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Joined: Jun 2006
Posts: 515 London, UK
Merlin
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Your signature made me laugh out loud rushes. I could hear Groucho saying that Well, thing is, I'm still havinhg problems. "The icons should NOT be lit up" But they are lit up. And when they're not, I get 0, and then the same 2 numbers from the first parameter test if I click both icons again to light them up. After finishing the first parameter test, am I meant to leave both icons lit up where it then says '100' before removing the filament?
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Re: Dracula Path of the Dragon - Filament test
[Re: Merlin]
#614838
04/16/10 12:10 PM
04/16/10 12:10 PM
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Joined: Jul 2005
Posts: 23,997 UK
Rushes
True Blue Boomer
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True Blue Boomer
Joined: Jul 2005
Posts: 23,997
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Gotta love Groucho.  We might have to wait for MaG's expert advice here, or a member who's played the game more recently and can recall this puzzle. My eyes are crossed just from reading the step-by-step. As a last resort, I have a saved game.
"Bleat, Watson -- unmitigated bleat!" ~ Sherlock Holmes
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Re: Dracula Path of the Dragon - Filament test
[Re: Rushes]
#614872
04/16/10 01:59 PM
04/16/10 01:59 PM
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Joined: Dec 1999
Posts: 69,977
MaG
Sonic Boomer
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Sonic Boomer
Joined: Dec 1999
Posts: 69,977
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Let me talk to myself here. First parameters: - you take a blank sample. Buzz it with the filament. Thus you tagged the monos and the polys (granulocytes). Read the monos using the left button and read the polys with the right button. You did that. Second parameter: - you take the sample that is in the filament that you already buzzed-tagged-counted granulocytes and return it to the blank sample in the tube. This way you mixed the buzzed with blanks to get an overall buzzed granulocytes percentage on that tube. When you took the filament off, that should have grayed the readout. Then you get a new sample of the mixed overall sample. Read the filament and get the total buzzed granulocytes. You might need to redo the first parameter and then continue to second parameter again.  I surely hope that I didn't confuse you further. Kruger's example is similar to tagging the cells to get the percentage.
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Re: Dracula Path of the Dragon - Filament test
[Re: MaG]
#614883
04/16/10 02:50 PM
04/16/10 02:50 PM
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Joined: Jun 2006
Posts: 515 London, UK
Merlin
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Hi MaG, "When you took the filament off, that should have grayed the readout." I'm still not doing something right. Example, sample 809. After checking the monos and polys separately (7 and 93) and then together to see '100' where both icons are lit simultaneously, do I leave them lit and just press the return button 'X' at the bottom right to come out of that screen, or do I make them both un-lit before coming out of that microscope screen? If I leave them un-lit, remove the filament from the microscope, and I proceed with mixing the sample and returning it to the microscope, then what I see is the icons un-lit, and no new number, just the same count of monos and polys again (7 and 93) when I click each icon. Or if I leave them lit, remove the filament from the microscope, and I proceed with mixing the sample and returning it to the microscope, then what I see is the icons lit and a new number. In this case, 24. Sorry, I don't get it.
Last edited by Merlin; 04/16/10 02:52 PM.
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Re: Dracula Path of the Dragon - Filament test
[Re: MaG]
#614906
04/16/10 03:40 PM
04/16/10 03:40 PM
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Joined: Jun 2006
Posts: 515 London, UK
Merlin
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Ok, I'll keep it within spoiler tags just in case. According to your walkthrough, "Total granulocytes is obtained by 100 (if all are marked in #3 at the readout above) x 100 (%) divided by the % marked granulocytes in readout #4."
So, does that mean 100x100 = 10000, then divided by 24? Which gives 416.6. But the total number of granulocytes per ml in the blood tests report is 0430, so I guess that's not right.
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Re: Dracula Path of the Dragon - Filament test
[Re: MaG]
#614969
04/16/10 07:10 PM
04/16/10 07:10 PM
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Joined: Jun 2006
Posts: 515 London, UK
Merlin
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Settled Boomer
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OP
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Joined: Jun 2006
Posts: 515
London, UK
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Hehe, yeah. it's Thanks for the help though MaG. One thing to mention to you, which may or may not be important for your walkthrough, I don't know. >> In your walkthrough, for samples 815, 812, and 810 you have written respectively 1428, 1428 and 1666 as the total granulocytes per 1000ml.
Because of the digit after the decimal point during my calculations was .5 or over, I put down 1429, 1429 and 1667. They all worked fine. So it seems either 1666 or 1667 work, and 1428 or 1429 both work.
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Re: Dracula Path of the Dragon - Filament test
[Re: MaG]
#619050
05/03/10 07:38 AM
05/03/10 07:38 AM
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Joined: Jul 2005
Posts: 23,997 UK
Rushes
True Blue Boomer
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True Blue Boomer
Joined: Jul 2005
Posts: 23,997
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starrs, you mention the decoded report. Did you already correct solve the first part of the decoding using the S E P T E letter code? That decodes the report -- but it starts off with UARC rather than YGRO.
The second part of the decoding is for the letter to Hans-Janos, and has the letter code A U G U S -- and that begins with YGRO.
Also check that your roman numerals are correctly set.
"Bleat, Watson -- unmitigated bleat!" ~ Sherlock Holmes
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Re: Dracula Path of the Dragon - Filament test
[Re: starrs4]
#619183
05/03/10 04:19 PM
05/03/10 04:19 PM
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Joined: Jul 2005
Posts: 23,997 UK
Rushes
True Blue Boomer
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True Blue Boomer
Joined: Jul 2005
Posts: 23,997
UK
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So you are trying to solve the first stage of the decoder puzzle - the S E P T E ? As I said in my last post, that should not begin with YGRO (as you mentioned trying to type in, in your above post), but with UARC. Did you use the pencil on the paper 3 times to reveal the full text? You might need to try a few more times with entering the letters for it to make any sense. Other members had the same problem, but found when they returned to the puzzle and tried again, the decoder worked correctly.
I also have a saved game if you would prefer that.
"Bleat, Watson -- unmitigated bleat!" ~ Sherlock Holmes
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Re: Dracula Path of the Dragon - Filament test
[Re: starrs4]
#619200
05/03/10 05:19 PM
05/03/10 05:19 PM
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Joined: Jul 2005
Posts: 23,997 UK
Rushes
True Blue Boomer
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True Blue Boomer
Joined: Jul 2005
Posts: 23,997
UK
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I just checked my saves - I don't have a labelled save for this puzzle so I'll send you a few from roughly this point, and hopefully one of them will be what you need. On the way to you now, with instructions on how to install. You're no trouble, we're happy to help. 
"Bleat, Watson -- unmitigated bleat!" ~ Sherlock Holmes
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